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primary human dermal microvascular endothelial cells hdmecs  (ATCC)
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ATCC primary human dermal microvascular endothelial cells hdmecs
Hypoosmotic stress activates OSR1 and SPAK through Piezo1. ( A ) Schematic of a cell responding to osmotic stress, either hyperosmotic or hypoosmotic, resulting in changes in membrane tension and Ca 2+ influx. ( B ) Immunoblot analysis of <t>HDMECs</t> treated with osmotic stress in the presence of DMSO or WNK463 (1 μM) for 5 min. The bar graph is presented as mean ± SD from three independent experiments. ** P < 0.01, *** P < 0.001 (unpaired two-tailed t test, nontreated vs. WNK463-treated); # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison test within the DMSO group). ( C ) siCTRL- and siPiezo1-treated HDMECs were exposed to osmotic stress for 5 min and analyzed by immunoblotting using the indicated antibodies. The results are expressed as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (siCTRL vs. siPiezo1). ( D and E ) Intracellular Ca 2+ levels were measured in Fluo-8 AM (2 µM)-labeled HDMECs exposed to isotonic, hypertonic (500 mM NaCl), or hypotonic [3:2 (v/v) deionized H 2 O/culture medium mixture] solutions, as indicated by the arrows. Data are expressed as mean ± SD (8 replicates, 2 independent experiments) ( D ). The graph represents the relative Fluo-8 AM intensity measured 3 min after osmotic stress exposure ( E ). Data are shown as mean ± SD. *** P < 0.001 (unpaired two-tailed t test, 8 replicates). ( F ) Ca 2+ -starved HDMECs, maintained in Ca 2+ /Mg 2+ -free HBSS (−Ca 2+ ) containing 0.1% FBS for 1 h, were exposed to osmotic stress in the Ca 2+ -free HBSS for 5 min. Cell lysates were analyzed by SDS-PAGE and immunoblotting. Data are shown as mean ± SD. ** P < 0.01; ns, not significant (n = 3). ( G ) Immunoblot analysis of HDMECs preincubated with DMSO or BAPTA-AM (10 μM) for 10 min, followed by exposure to osmotic stress for 5 min. The results are presented as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (DMSO-treated vs. BAPTA-AM-treated).
Primary Human Dermal Microvascular Endothelial Cells Hdmecs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary human dermal microvascular ecs  (PromoCell)
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PromoCell primary human dermal microvascular ecs
Hypoosmotic stress activates OSR1 and SPAK through Piezo1. ( A ) Schematic of a cell responding to osmotic stress, either hyperosmotic or hypoosmotic, resulting in changes in membrane tension and Ca 2+ influx. ( B ) Immunoblot analysis of <t>HDMECs</t> treated with osmotic stress in the presence of DMSO or WNK463 (1 μM) for 5 min. The bar graph is presented as mean ± SD from three independent experiments. ** P < 0.01, *** P < 0.001 (unpaired two-tailed t test, nontreated vs. WNK463-treated); # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison test within the DMSO group). ( C ) siCTRL- and siPiezo1-treated HDMECs were exposed to osmotic stress for 5 min and analyzed by immunoblotting using the indicated antibodies. The results are expressed as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (siCTRL vs. siPiezo1). ( D and E ) Intracellular Ca 2+ levels were measured in Fluo-8 AM (2 µM)-labeled HDMECs exposed to isotonic, hypertonic (500 mM NaCl), or hypotonic [3:2 (v/v) deionized H 2 O/culture medium mixture] solutions, as indicated by the arrows. Data are expressed as mean ± SD (8 replicates, 2 independent experiments) ( D ). The graph represents the relative Fluo-8 AM intensity measured 3 min after osmotic stress exposure ( E ). Data are shown as mean ± SD. *** P < 0.001 (unpaired two-tailed t test, 8 replicates). ( F ) Ca 2+ -starved HDMECs, maintained in Ca 2+ /Mg 2+ -free HBSS (−Ca 2+ ) containing 0.1% FBS for 1 h, were exposed to osmotic stress in the Ca 2+ -free HBSS for 5 min. Cell lysates were analyzed by SDS-PAGE and immunoblotting. Data are shown as mean ± SD. ** P < 0.01; ns, not significant (n = 3). ( G ) Immunoblot analysis of HDMECs preincubated with DMSO or BAPTA-AM (10 μM) for 10 min, followed by exposure to osmotic stress for 5 min. The results are presented as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (DMSO-treated vs. BAPTA-AM-treated).
Primary Human Dermal Microvascular Ecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human dermal microvascular ecs/product/PromoCell
Average 96 stars, based on 1 article reviews
primary human dermal microvascular ecs - by Bioz Stars, 2026-02
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primary human dermal microvascular endothelial cells  (PromoCell)
96
PromoCell primary human dermal microvascular endothelial cells
Effect of SGE on the barrier function of human dermal endothelium. Error bars represent the SEM. <t>Trans-endothelial</t> electrical resistance (TEER) of primary human dermal <t>microvascular</t> endothelial cells (DMECs) grown on the apical side of a transwell insert was measured after stimulation of DMECs with SGE (1 SGP per well) or TNF-α (100 ng/mL) as a positive control. Data pooled from three individual experiments, three transwells per condition, each measured three times per timepoint. Statistically significant differences between SGE and mock are indicated with an asterisk * p < 0.05; as measured by 2way ANOVA multiple comparisons (A) DMECs were stimulated with SGE from either Ae. aegypti , Ae. albopictus , Ae. japonicus , or (B) Cx. pipiens molestus or Cx. pipiens pipiens . The %TEER was measured every 3 h over a time-course of 12 h, and again at 24 and 48 h post-stimulation.
Primary Human Dermal Microvascular Endothelial Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human dermal microvascular endothelial cells/product/PromoCell
Average 96 stars, based on 1 article reviews
primary human dermal microvascular endothelial cells - by Bioz Stars, 2026-02
96/100 stars
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Hypoosmotic stress activates OSR1 and SPAK through Piezo1. ( A ) Schematic of a cell responding to osmotic stress, either hyperosmotic or hypoosmotic, resulting in changes in membrane tension and Ca 2+ influx. ( B ) Immunoblot analysis of HDMECs treated with osmotic stress in the presence of DMSO or WNK463 (1 μM) for 5 min. The bar graph is presented as mean ± SD from three independent experiments. ** P < 0.01, *** P < 0.001 (unpaired two-tailed t test, nontreated vs. WNK463-treated); # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison test within the DMSO group). ( C ) siCTRL- and siPiezo1-treated HDMECs were exposed to osmotic stress for 5 min and analyzed by immunoblotting using the indicated antibodies. The results are expressed as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (siCTRL vs. siPiezo1). ( D and E ) Intracellular Ca 2+ levels were measured in Fluo-8 AM (2 µM)-labeled HDMECs exposed to isotonic, hypertonic (500 mM NaCl), or hypotonic [3:2 (v/v) deionized H 2 O/culture medium mixture] solutions, as indicated by the arrows. Data are expressed as mean ± SD (8 replicates, 2 independent experiments) ( D ). The graph represents the relative Fluo-8 AM intensity measured 3 min after osmotic stress exposure ( E ). Data are shown as mean ± SD. *** P < 0.001 (unpaired two-tailed t test, 8 replicates). ( F ) Ca 2+ -starved HDMECs, maintained in Ca 2+ /Mg 2+ -free HBSS (−Ca 2+ ) containing 0.1% FBS for 1 h, were exposed to osmotic stress in the Ca 2+ -free HBSS for 5 min. Cell lysates were analyzed by SDS-PAGE and immunoblotting. Data are shown as mean ± SD. ** P < 0.01; ns, not significant (n = 3). ( G ) Immunoblot analysis of HDMECs preincubated with DMSO or BAPTA-AM (10 μM) for 10 min, followed by exposure to osmotic stress for 5 min. The results are presented as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (DMSO-treated vs. BAPTA-AM-treated).

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Activation of WNK1 signaling through Piezo1

doi: 10.1073/pnas.2513155122

Figure Lengend Snippet: Hypoosmotic stress activates OSR1 and SPAK through Piezo1. ( A ) Schematic of a cell responding to osmotic stress, either hyperosmotic or hypoosmotic, resulting in changes in membrane tension and Ca 2+ influx. ( B ) Immunoblot analysis of HDMECs treated with osmotic stress in the presence of DMSO or WNK463 (1 μM) for 5 min. The bar graph is presented as mean ± SD from three independent experiments. ** P < 0.01, *** P < 0.001 (unpaired two-tailed t test, nontreated vs. WNK463-treated); # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison test within the DMSO group). ( C ) siCTRL- and siPiezo1-treated HDMECs were exposed to osmotic stress for 5 min and analyzed by immunoblotting using the indicated antibodies. The results are expressed as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (siCTRL vs. siPiezo1). ( D and E ) Intracellular Ca 2+ levels were measured in Fluo-8 AM (2 µM)-labeled HDMECs exposed to isotonic, hypertonic (500 mM NaCl), or hypotonic [3:2 (v/v) deionized H 2 O/culture medium mixture] solutions, as indicated by the arrows. Data are expressed as mean ± SD (8 replicates, 2 independent experiments) ( D ). The graph represents the relative Fluo-8 AM intensity measured 3 min after osmotic stress exposure ( E ). Data are shown as mean ± SD. *** P < 0.001 (unpaired two-tailed t test, 8 replicates). ( F ) Ca 2+ -starved HDMECs, maintained in Ca 2+ /Mg 2+ -free HBSS (−Ca 2+ ) containing 0.1% FBS for 1 h, were exposed to osmotic stress in the Ca 2+ -free HBSS for 5 min. Cell lysates were analyzed by SDS-PAGE and immunoblotting. Data are shown as mean ± SD. ** P < 0.01; ns, not significant (n = 3). ( G ) Immunoblot analysis of HDMECs preincubated with DMSO or BAPTA-AM (10 μM) for 10 min, followed by exposure to osmotic stress for 5 min. The results are presented as mean ± SD. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3), unpaired two-tailed t test (DMSO-treated vs. BAPTA-AM-treated).

Article Snippet: Primary human dermal microvascular endothelial cells (HDMECs) (CRL-3243, ATCC, Manassas, VA) were grown in complete MCDB 131 medium (15-100-CV, Corning, Corning, NY) supplemented with 10% FBS (F0926, MilliporeSigma, St Louis, MO), 1% L-glutamine (25-005-CI, Corning), 1% penicillin/streptomycin (SV30010, Thermo Fisher Scientific, Waltham, MA), 1 μg/mL hydrocortisone (H0888, MilliporeSigma), and 10 ng/mL epidermal growth factor (PHG0311, Thermo Fisher Scientific).

Techniques: Membrane, Western Blot, Two Tailed Test, Comparison, Labeling, SDS Page

Effect of SGE on the barrier function of human dermal endothelium. Error bars represent the SEM. Trans-endothelial electrical resistance (TEER) of primary human dermal microvascular endothelial cells (DMECs) grown on the apical side of a transwell insert was measured after stimulation of DMECs with SGE (1 SGP per well) or TNF-α (100 ng/mL) as a positive control. Data pooled from three individual experiments, three transwells per condition, each measured three times per timepoint. Statistically significant differences between SGE and mock are indicated with an asterisk * p < 0.05; as measured by 2way ANOVA multiple comparisons (A) DMECs were stimulated with SGE from either Ae. aegypti , Ae. albopictus , Ae. japonicus , or (B) Cx. pipiens molestus or Cx. pipiens pipiens . The %TEER was measured every 3 h over a time-course of 12 h, and again at 24 and 48 h post-stimulation.

Journal: Emerging Microbes & Infections

Article Title: Effect of mosquito saliva from distinct species on human dermal endothelial cell function in vitro and West Nile virus pathogenesis in vivo

doi: 10.1080/22221751.2025.2502006

Figure Lengend Snippet: Effect of SGE on the barrier function of human dermal endothelium. Error bars represent the SEM. Trans-endothelial electrical resistance (TEER) of primary human dermal microvascular endothelial cells (DMECs) grown on the apical side of a transwell insert was measured after stimulation of DMECs with SGE (1 SGP per well) or TNF-α (100 ng/mL) as a positive control. Data pooled from three individual experiments, three transwells per condition, each measured three times per timepoint. Statistically significant differences between SGE and mock are indicated with an asterisk * p < 0.05; as measured by 2way ANOVA multiple comparisons (A) DMECs were stimulated with SGE from either Ae. aegypti , Ae. albopictus , Ae. japonicus , or (B) Cx. pipiens molestus or Cx. pipiens pipiens . The %TEER was measured every 3 h over a time-course of 12 h, and again at 24 and 48 h post-stimulation.

Article Snippet: Primary Human Dermal Microvascular Endothelial cells (DMECs; Cell Systems, ACBRI 538) were maintained at 37°C, 5% CO 2 in MV2 medium (Promocell, C-22121).

Techniques: Positive Control